Journal: European Journal of Medical Research

Article Title: XBP1-driven proliferative B cell subcluster in Diffuse Large B Cell Lymphoma linked to altered nucleotide metabolism

doi: 10.1186/s40001-025-03673-2

Figure Lengend Snippet: Single-cell landscape in DLBCL. A UMAP plot annotating 6 main cell clusters (B cells, CD8+ T cells, myeloid cells, naïve T cells, NK cells, and Tregs) based on the dataset GSE182434 . B Bubble plot showing the expression levels of the marker genes specific to each cell cluster. C , D Percentage of the 6 main cell clusters in the samples of DLBCL and the control.

Article Snippet: Prior to the laboratory validation assays, the DLBCL cell lines U2932 (code no. CL-0838) and SU-DHL-10 (code no. CL-0758) were ordered from Procell (Wuhan, China, https://www.procell.com.cn/ ) and cultured in the Roswell Park Memorial Institute-1640 medium (PM150110, Procell, China) supplemented with 10% fetal bovine serum (164210, Procell, China) and 1% penicillin/streptomycin ( PB180120 , Wuhan, China).

Techniques: Single Cell, Expressing, Marker, Control

Journal: European Journal of Medical Research

Article Title: XBP1-driven proliferative B cell subcluster in Diffuse Large B Cell Lymphoma linked to altered nucleotide metabolism

doi: 10.1186/s40001-025-03673-2

Figure Lengend Snippet: Copy number variation analysis in B cells of DLBCL. A Heatmap demonstrating the copy number variation analysis in B cells. B-C Enrichment analysis on the genes belonging to the amplification region Chr1q ( B ) and the deletion region Chr6 ( C ).

Article Snippet: Prior to the laboratory validation assays, the DLBCL cell lines U2932 (code no. CL-0838) and SU-DHL-10 (code no. CL-0758) were ordered from Procell (Wuhan, China, https://www.procell.com.cn/ ) and cultured in the Roswell Park Memorial Institute-1640 medium (PM150110, Procell, China) supplemented with 10% fetal bovine serum (164210, Procell, China) and 1% penicillin/streptomycin ( PB180120 , Wuhan, China).

Techniques: Amplification

Journal: European Journal of Medical Research

Article Title: XBP1-driven proliferative B cell subcluster in Diffuse Large B Cell Lymphoma linked to altered nucleotide metabolism

doi: 10.1186/s40001-025-03673-2

Figure Lengend Snippet: Identification of proliferation-related B cell subclusters in DLBCL. A UMAP plot showing B cell subclusters (subcluster 1–4). B Percentage of each cell subcluster in the samples of DLBCL and the control. C Violin plot showing the expression levels of marker genes specific to each subcluster. D Enrichment analysis on the enriched pathways of differential genes belonging to each subcluster.

Article Snippet: Prior to the laboratory validation assays, the DLBCL cell lines U2932 (code no. CL-0838) and SU-DHL-10 (code no. CL-0758) were ordered from Procell (Wuhan, China, https://www.procell.com.cn/ ) and cultured in the Roswell Park Memorial Institute-1640 medium (PM150110, Procell, China) supplemented with 10% fetal bovine serum (164210, Procell, China) and 1% penicillin/streptomycin ( PB180120 , Wuhan, China).

Techniques: Control, Expressing, Marker

Journal: European Journal of Medical Research

Article Title: XBP1-driven proliferative B cell subcluster in Diffuse Large B Cell Lymphoma linked to altered nucleotide metabolism

doi: 10.1186/s40001-025-03673-2

Figure Lengend Snippet: SCENIC analysis on B cell subcluster 2. A Difference in the AUCell score in B cells of both DLBCL and control samples. B – E Enrichment analysis on the downstream targets of transcription factors XBP1 ( B ), SPIB ( C ), RELB ( D ), and IRF ( E ). ns p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001

Article Snippet: Prior to the laboratory validation assays, the DLBCL cell lines U2932 (code no. CL-0838) and SU-DHL-10 (code no. CL-0758) were ordered from Procell (Wuhan, China, https://www.procell.com.cn/ ) and cultured in the Roswell Park Memorial Institute-1640 medium (PM150110, Procell, China) supplemented with 10% fetal bovine serum (164210, Procell, China) and 1% penicillin/streptomycin ( PB180120 , Wuhan, China).

Techniques: Control

Journal: Leukemia & lymphoma

Article Title: CD40 ligand is necessary and sufficient to support primary diffuse large B-cell lymphoma cells in culture: a tool for in vitro preclinical studies with primary B-cell malignancies

doi: 10.3109/10428194.2011.654337

Figure Lengend Snippet: Expression of CD40 in primary dog B cell lymphomas. (A) A heat map of selected gene expression in dog B-cell and T-cell lymphomas. Expressions of genes, chosen for prototypical expression in B-cells and T-cells, were shown as a heat map where color represents fluorescence intensity of hybridized probes as indicated at the bottom and therefore the amount of gene transcript in any given sample. Generally, Red/Orange represents high expression, Yellow/Green represents moderate expression and Blue/Black represents little or no expression. (B) DLBCL samples were stained for CD22 and CD40 (using CD40L-CD8 fusion protein). A representative result using an isotype control antibody and streptavidin (SA control) is shown as a negative control (left panel). One representative sample of 3 different primary dog B-cell lymphomas is shown, including the mean fluorescence intensity (MFI) of the FITC channel.

Article Snippet: The human DLBCL cell line SU-DHL4 was obtained from Dr. Lee Honigberg (Pharmacyclics Inc., Sunnyvale, CA).

Techniques: Expressing, Gene Expression, Fluorescence, Staining, Control, Negative Control

Journal: Leukemia & lymphoma

Article Title: CD40 ligand is necessary and sufficient to support primary diffuse large B-cell lymphoma cells in culture: a tool for in vitro preclinical studies with primary B-cell malignancies

doi: 10.3109/10428194.2011.654337

Figure Lengend Snippet: In vitro culture of primary dog DLBCL cells with KtCD40L. (A) Photomicrographs of DLBCL cells alone (left), DLBCL cells cultured with KtCD40L (middle), and KtCD40L alone (right), each shown after seven days in culture. Bars = 100 μm (B) Flow analysis of DLBCL cells before and after culture with KtCD40L for 7 days. Estimates of cell size and complexity were obtained from flow cytometric light scatter properties (upper panels). Viability was determined by 7-AAD exclusion (lower panels). FSC; forward scatter, SSC; side scatter (C) Growth curves of primary dog DLBCL cells (n = 4) cultured with KtCD40L. DLBCL cell numbers were determined at each time point using hemacytometer with dead cell exclusion by trypan blue staining.

Article Snippet: The human DLBCL cell line SU-DHL4 was obtained from Dr. Lee Honigberg (Pharmacyclics Inc., Sunnyvale, CA).

Techniques: In Vitro, Cell Culture, Staining

Journal: Leukemia & lymphoma

Article Title: CD40 ligand is necessary and sufficient to support primary diffuse large B-cell lymphoma cells in culture: a tool for in vitro preclinical studies with primary B-cell malignancies

doi: 10.3109/10428194.2011.654337

Figure Lengend Snippet: Phenotypic characteristics of DLBCL cells cultured with KtCD40L. (A) DLBCL cells cultured with KtCD40L for 48 days were phenotyped using flow cytometry. Gray lines represent unstained samples. One representative sample of three DLBCL cases analyzed is shown. (B) Clonal IgH gene rearrangements of the same size (105 bp) were observed in tumor cells before (Day 0) and after (Day 48) the culture. One representative sample of two DLBCL cases analyzed is shown. (C) Examples of FISH images obtained from one DLBCL case with trisomy of CFA 31. Control dog metaphase chromosomes (a) and an interphase nucleus (b) probed with a marker for CFA 31 (yellow signal), exhibiting the expected chromosomal location and normal copy number status (n = 2) of this region. Three copies of this locus were evident in 51% of DLBCL cells prior to culture (c), and in 48% of DLBCL cells after culture with KtCD40L for 40 days (d). A KtCD40L cell (upper left without a probe signal) and a DLBCL cell (lower right with probe signals) are shown together in (d).

Article Snippet: The human DLBCL cell line SU-DHL4 was obtained from Dr. Lee Honigberg (Pharmacyclics Inc., Sunnyvale, CA).

Techniques: Cell Culture, Flow Cytometry, Control, Marker

Journal: Leukemia & lymphoma

Article Title: CD40 ligand is necessary and sufficient to support primary diffuse large B-cell lymphoma cells in culture: a tool for in vitro preclinical studies with primary B-cell malignancies

doi: 10.3109/10428194.2011.654337

Figure Lengend Snippet: Feeder cell-free culture of primary DLBCL cells with recombinant shuCD40L. (A) The MTS cell proliferation assay was performed at 24-hour intervals on primary dog DLBCL cells stimulated with increasing amounts of shuCD40L. Data from 4 independent primary dog DLBCL samples are shown. Data were normalized to results from 5 × 104 cells (0 hour) to show fold increase of cell numbers during the culture period. (B) MTS cell proliferation assay was performed on primary DLBCL cells stimulated with 100 ng/mL shuCD40L in the presence of increasing amounts of anti-human CD40L-IgA. Three different primary DLBCL samples were tested for 72 hours in duplicate conditions and data were normalized to results of cells cultured with 100 ng/mL shuCD40L without neutralizing antibodies. Data shown represent the mean ± SD (n = 3) and statistical significance was tested against the condition of 100 ng/mL shuCD40L without neutralizing antibodies using ANOVA (*P < 0.01, **P < 0.001). (C) Human B-ALL samples were stained for CD22 and CD40. CD22+ tumor cells (> 90% CD22+ cells in both samples) were gated and CD40 expression was analyzed. Gray lines represent staining with an isotype control antibody. (D) The MTS cell proliferation assay using primary human B-ALL cells was performed as described above. Data from 2 independent samples are shown.

Article Snippet: The human DLBCL cell line SU-DHL4 was obtained from Dr. Lee Honigberg (Pharmacyclics Inc., Sunnyvale, CA).

Techniques: Recombinant, Proliferation Assay, Cell Culture, Staining, Expressing, Control

Journal: Leukemia & lymphoma

Article Title: CD40 ligand is necessary and sufficient to support primary diffuse large B-cell lymphoma cells in culture: a tool for in vitro preclinical studies with primary B-cell malignancies

doi: 10.3109/10428194.2011.654337

Figure Lengend Snippet: IC 50 of 22KDEL and Bic3 for tumor cells

Article Snippet: The human DLBCL cell line SU-DHL4 was obtained from Dr. Lee Honigberg (Pharmacyclics Inc., Sunnyvale, CA).

Techniques:

Journal: Leukemia & lymphoma

Article Title: CD40 ligand is necessary and sufficient to support primary diffuse large B-cell lymphoma cells in culture: a tool for in vitro preclinical studies with primary B-cell malignancies

doi: 10.3109/10428194.2011.654337

Figure Lengend Snippet: Comparable assessment of cytotoxicity using dog and human B-cell malignancies. (A) Four independent primary dog DLBCL samples; #1–3 are the same as in Figure 4, #4 is from a different dog, and (B) two human primary B-ALL samples from different patients than those shown in Figure 4, were cultured for 72 hours with a log serial dilutions (0.01–100 nM) of 22KDEL and Bic3 immunotoxins in the presence of shuCD40L. Experiments were performed in duplicate and cell numbers were determined using the MTS assay. Results with 10 nM immunotoxins are shown, and a summary result of the IC50 for 22KDEL and Bic3 is shown in Table I.

Article Snippet: The human DLBCL cell line SU-DHL4 was obtained from Dr. Lee Honigberg (Pharmacyclics Inc., Sunnyvale, CA).

Techniques: Cell Culture, MTS Assay